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Validation of shRNA clones for gene silencing in 293FT cells

von Wen Wang

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[1.] Ww/Fragment 001 13 - Diskussion
Zuletzt bearbeitet: 2014-10-28 02:51:08 Hindemith
Fragment, Gesichtet, SMWFragment, Schutzlevel sysop, Verschleierung, Wikipedia Small interfering RNA 2007, Ww

Typus
Verschleierung
Bearbeiter
SleepyHollow02
Gesichtet
Yes
Untersuchte Arbeit:
Seite: 1, Zeilen: 13-21
Quelle: Wikipedia Small interfering RNA 2007
Seite(n): 1 (Internetquelle), Zeilen: -
1.1.2 siRNA

Small interfering RNAs (siRNAs), sometimes known as short interfering RNA or silencing RNAs, represent a class of 20-25 nucleotide-long double-stranded RNA molecules that play a variety of roles in biology.

siRNAs have a well defined structure: a short (usually 21-nt) double-strand of RNA (dsRNA) with 2-nt 3' overhangs on either end. Each strand has a 5' phosphate group and a 3' hydroxyl (-OH) group. This structure is the result of processing by Dicer, an enzyme that converts either long dsRNA or hairpin RNAs into siRNAs (Baulcombe et al, 1999).

Most notably, siRNAs are involved in the RNA interference (RNAi) pathway [where the siRNA interferes with the expression of a specific gene (Tuschl et al, 2001).]

Small interfering RNA (siRNA), sometimes known as short interfering RNA or silencing RNA, are a class of 20-25 nucleotide-long double-stranded RNA molecules that play a variety of roles in biology. Most notably, siRNA is involved in the RNA interference (RNAi) pathway where the siRNA interferes with the expression of a specific gene. [...]

[...]

SiRNAs have a well defined structure: a short (usually 21-nt) double-strand of RNA (dsRNA) with 2-nt 3' overhangs on either end:

[...]

Each strand has a 5' phosphate group and a 3' hydroxyl (-OH) group. This structure is the result of processing by dicer, an enzyme that converts either long dsRNAs or small hairpin RNAs into siRNAs.[3]


3. Bernstein E, Caudy A, Hammond S, Hannon G (2001). "Role for a bidentate ribonuclease in the initiation step of RNA interference". Nature 409 (6818): 363–6. PMID 11201747.

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Sichter
(SleepyHollow02), Hindemith



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