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Validation of shRNA clones for gene silencing in 293FT cells

von Wen Wang

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[1.] Ww/Fragment 024 11 - Diskussion
Zuletzt bearbeitet: 2014-10-28 03:02:44 Hindemith
Fragment, Gesichtet, SMWFragment, Schutzlevel sysop, Verschleierung, Wikipedia HEK 293 cells 2007, Ww

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Verschleierung
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SleepyHollow02
Gesichtet
Yes
Untersuchte Arbeit:
Seite: 24, Zeilen: 11-21
Quelle: Wikipedia HEK 293 cells 2007
Seite(n): 1 (Internetquelle), Zeilen: -
1.4 293FT cell lines

Human Embryonic Kidney cells, also known as HEK cells, HEK 293 or just 293 cells, are a cell line originally derived, as their name indicates, from embryonic human kidney. HEK cells are very easy to grow and transfect very readily and so are widely-used in cell biology research. They are also used by biotechnology industry to produce therapeutic proteins and viruses for gene therapy.

1.4.1 Origins of 293 cells

293 cells were generated by transformation of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA in the laboratory of Alex Van der Eb in Leiden, Holland in the early 1970s. They are called HEK for human embryonic kidney, while the number 293 roots from numbering of experiments

Human Embryonic Kidney cells, also known as HEK cells, HEK 293 or just 293 cells, are a cell line originally derived, as their name indicates, from embryonic human kidney. HEK cells are not themselves particularly interesting, but are very easy to work with, and so are a widely-used cell line in cell biology research. [...]

[...]

HEK 293 cells were generated by transformation of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA in the laboratory of Alex Van der Eb in Leiden, Holland in the early 70s.

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