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Untersuchte Arbeit: Seite: 11, Zeilen: 25-30 |
Quelle: Hammond 2005 Seite(n): 5825, Zeilen: r.col. 14 ff. |
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Chromatographic purification of RISC nuclease activity from Drosophila cells revealed several RISC components. The first identified component was Argonaute2 (Ago2) (Hammond et al, 2001). This protein is a member of a gene family conserved in most eukaryotic and several prokaryotic genomes. The C. elegans homolog, rde-1, was previously identified in a genetic screen for RNAi-deficient mutants, reinforcing its connection with RNAi (Tabara et al, [1999).] | Chromatographic purification of RISC nuclease activity from Drosophila cells revealed several RISC components. The first identified component was Argonaute2 (Ago2) [43]. This protein is a member of a gene family conserved in most eukaryotic and several prokaryotic genomes. The C. elegans homolog, rde-1, was previously identified in a genetic screen for RNAi-deficient mutants, reinforcing its connection with RNAi [12].
[12] Tabara, H., Sarkissian, M., Kelly, W.G., Fleenor, J., Grishok, A., Timmons, L., Fire, A. and Mello, C.C. (1999) The rde-1 gene, RNA interference, and transposon silencing in C. elegans. Cell 99, 123–132. [43] Hammond, S.M., Boettcher, S., Caudy, A.A., Kobayashi, R. and Hannon, G.J. (2001) Argonaute2, a link between genetic and biochemical analyses of RNAi. Science 293, 1146–1150 |
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