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SleepyHollow02
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Untersuchte Arbeit:
Seite: 25, Zeilen: 24-29
Quelle: Wikipedia HEK 293 cells 2007
Seite(n): 1 (Internetquelle), Zeilen: -
Subsequent analysis has shown that the transformation was brought about by an insert consisting of -4.5 kilo bases from the left arm of the viral genome, which became incorporated into human chromosome 19 (Louis et al, 1997).

For many years it was assumed that HEK 293 cells were generated by transformation of either a fibroblastic, endothelial or epithelial cell all of which are abundant in kidney. However the fact that the cells originated from [cultured kidney cells does not clearly indicate the exact cellular origin of the HEK 293, as embryonic kidney cultures may contain small numbers of almost all cell types of the body.]

Subsequent analysis has shown that the transformation was brought about by an insert consisting of ~4.5 kilobases from the left arm of the viral genome, which became incorporated into human chromosome 19 (Louis 1997[2]).

For many years it was assumed that HEK 293 cells were generated by transformation of either a fibroblastic, endothelial or epithelial cell all of which are abundant in kidney. However the fact that the cells originated from cultured kidney cells does not say much about the exact cellular origin of the HEK 293, as embryonic kidney cultures may contain small numbers of almost all cell types of the body.

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