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Verschleierung
Bearbeiter
Hindemith
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Untersuchte Arbeit:
Seite: 29, Zeilen: 3-11
Quelle: Root et al 2006
Seite(n): 716, Zeilen: figure 1
Ww 29a diss

Fig. 2. Map for the pLKO.1 lentiviral vector. It derives from pRRLSIN.cPPT.PGK/GFP/WRPE and contains elements for efficient viral packaging and shRNA expression. These include Ψ, the lentiviral packaging site; RRE, the Rev-responsive element; cPPT. RSV/5’LTR is a hybrid of the Rous Sarcoma virus promoter and the HIV 5’LTR. SIN 3’LTR is the HIV 3’LTR with a self-inactivating U3 deletion. Expression of the shRNA is driven by the human U6 promoter (hU6). The lentiviral vector also contains the mammalian selection marker puromycin resistance gene (PAC) under the control of the PGK promoter as a mammalian selection marker. pUCori is the bacterial origin of replication of the plasmid, F1 ori is the single-stranded phage F1 origin of replication, and AmpR is the ampicillin resistance gene.

Ww 29a source

Figure 1 Vector map for the pLKO.1 lentiviral vector. The self-inactivating lentiviral vector backbone contains elements for efficient viral packaging and shRNA expression. These include Ψ, the lentiviral packaging site; RRE, the Rev-responsive element; cPPT. RSV/ 5′ LTR is a hybrid of the Rous Sarcoma virus promoter and the HIV 5′ LTR. SIN 3′ LTR is the HIV 3′ LTR with a self-inactivating U3 deletion. Expression of the shRNA is driven by the human U6 promoter (hU6). The lentiviral vector also contains the mammalian selection marker puromycin resistance gene (PAC) under the control of the PGK promoter as a mammalian selection marker. pUCori is the bacterial origin of replication of the plasmid, F1 ori is the single-stranded phage F1 origin of replication, and AmpR is the ampicillin resistance gene.

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