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Untersuchte Arbeit: Seite: 41, Zeilen: 11-17 |
Quelle: Kilic and Yuan 2007 Seite(n): 1 (online source), Zeilen: 0028 |
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The Midi plasmid purification protocols were based on a modified alkaline lysis procedure, followed by binding of plasmid DNA to Anion-Exchange Resin under appropriate low-salt and pH conditions. RNA, proteins, dyes, and low-molecular-weight impurities were removed by a medium-salt wash. Plasmid DNA was eluted in a high salt buffer and then concentrated and desalted by isopropanol precipitation. | This plasmid preparation protocol used in this embodiment is based on a modified alkaline lysis procedure, followed by binding of plasmid DNA to anion-exchange resin under appropriate low-salt and pH conditions. RNA, proteins, dyes, and low-molecular-weight impurities in the plasmid are removed by a medium-salt wash. Plasmid DNA is eluted in a high-salt buffer and then concentrated and desalted by isopropanol precipitation. |
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