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Typus
Verschleierung
Bearbeiter
Hindemith
Gesichtet
Yes
Untersuchte Arbeit:
Seite: 41, Zeilen: 11-17
Quelle: Kilic and Yuan 2007
Seite(n): 1 (online source), Zeilen: 0028
The Midi plasmid purification protocols were based on a modified alkaline lysis procedure, followed by binding of plasmid DNA to Anion-Exchange Resin under appropriate low-salt and pH conditions. RNA, proteins, dyes, and low-molecular-weight impurities were removed by a medium-salt wash. Plasmid DNA was eluted in a high salt buffer and then concentrated and desalted by isopropanol precipitation. This plasmid preparation protocol used in this embodiment is based on a modified alkaline lysis procedure, followed by binding of plasmid DNA to anion-exchange resin under appropriate low-salt and pH conditions. RNA, proteins, dyes, and low-molecular-weight impurities in the plasmid are removed by a medium-salt wash. Plasmid DNA is eluted in a high-salt buffer and then concentrated and desalted by isopropanol precipitation.
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Sichter
(Hindemith) Schumann